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With Dr. Lamberto Re from Italy Ancona
Dr. Lamberto Re’s research blew my mind and I would like to recap in simple words what he discovered:
How ozone works is the most frequently asked question we have received. Ozone is a free radical so why do we choose it as a healing agent? Shouldn’t we avoid free radicals and rather take antioxidants?
As seen in the graph below Oxidative Stress has implication in all organs. Many diseases are related to high oxidative stress.
With chronic oxidative stress we have a deficit of SOD (superoxide dismutase), GPX (glutathione peroxidase) and CAT (catalase) as the major anti-oxidant enzymes.
Ozone Therapy is administered through various different routes and with variable therapeutic doses. It is administered in the form of gas. Ozone represents an extremely unstable molecule characterized by 3 atoms of oxygen. When it comes in contact with fluid within the body or organic matter (cells, bacteria, viruses, blood…) Ozonolysis takes place. This process happens in fractions of seconds.
The reaction leads to the formation of:
Ozonides, Aldehydes and Peroxides (hydrogen peroxide – H2O2).
Ozonides, Aldehydes and Hydrogen Peroxide (H2O2) induce a mild oxidative stress at cellular level whereby H2O2 is quickly reduced to H2O (water) and O2 (oxygen). The half-life of H2O2 is literally 60 seconds and yet its intracellular concentration is critical in order to activate all relevant biochemical pathways. Thus H2O2 causes an oxidative reaction, which triggers secondary messengers with long lasting action.
Only small amounts of the anti-oxidant capacity of the blood are required to reduce Hydrogen peroxide to water (H20). In 20 minutes the potent anti-oxidant capacity of the blood is fully reconstituted owing to the efficiency of the redox system. No damage to any cells occurs during this process.
The paradoxical concept that ozone induces an antioxidant response capable of reversing chronic oxidative stress is common in the animal and vegetal kingdom.
We can therefore recapitulate that repetitive brief oxidative stresses induced by Ozone activates the cells defences against the deleterious action of the reactive oxygen species (ROS). Ozone can therefore be defined as a conditioning agent that activates a cascade of signaling systems, which in turn help our body to heal itself. This fact is now supported by findings of an increased level of antioxidant enzymes during and after Ozone therapy, which brings benefits in many pathological conditions.
Repeated oxidative stress caused by Ozone causes the production of Nrf2 protein, which is able to initiate the production of proteins favouring most of the cell functions.
Nrf2 is a powerful protein that is latent in every cell. It is a switch for 200 or more different genes, all of which code for different aspects of cellular defense such as immune and inflammatory responses, tissue remodelling, fibrosis, carcinogenesis metastasis, and even cognitive dysfunction and addictive behaviour.
Nrf2 is unable to operate until a potent Nrf2 activator like Ozone releases it. Once released it migrates into the cell nucleus and bonds to the DNA at the location of the Human Antioxidant Response Element (hARE) which is the master regulator of the total antioxidant system that is available in ALL human cells.
When Nrf2 is activated in the nucleus, it turns on the production of antioxidant enzymes such as Catalase, Glutathione Peroxidase and Superoxide Dismutase (SOD) our most powerful neutralizers of free radicals that our body produces.
Biological responses induced via the activation of Nrf2 are:
To put it simply, the Nrf2-dependent anti-oxidant response has been shown to protect against oxidative stress related diseases such as cancer, neurodegenerative diseases (Alzheimer, Parkinson, ALS, Huntington’s disease), cardiovascular disease, infections, lung emphysema, inflammation and aging.
Dr. Lamberto Re says:
“The results of our study show for the first time in vivo that ozone increases levels of Nrf2 protein, which in turn promotes the antioxidant and detoxifying enzymes of phase II. Furthermore, a significant increase of SOD and CAT enzymes has been observed at the end of the treatment. “
First an NrF2 control was taken by measuring the levels in the blood of the patient (T1).
The second bar (T2) shows the NrF2 levels during major autohemo therapy in the 100ml flask after infusing the blood with ozone. Then the blood was dripped back into the body.
Exactly 1/2 hour later Dr. Lamberto took a small probe out of the 7 l blood from the patient and measured the NrF2 levels (T3). The result was almost identical as it was in the 100ml in the flask. This means that any amount of Ozone that is induced into the body triggers a systemic reaction. All cells are activated with NrF2 and the body develops a systemic healing response.
This has been proven by our dear friend and teacher Dr. Lamberto Re from Ancona Italy. In an experiment he used the blood of patients to show that the amount of ozone administered to a body has little to do with the systemic reaction. That’s why small homeopathic doses as well as mega multiples doses of ozone show all good results. Ozone acts as a switch that turns on a cascade of systemic reactions that proof to be very favourable to recovery and healing.
Re, L. et al., 2014. Is ozone pre-conditioning effect linked to Nrf2/EpRE activation pathway in vivo? A preliminary result. European Journal of Pharmacology, 742, pp.158–162. Available at: https://linkinghub.elsevier.com/retrieve/pii/S0014299914006347.
Viebahn-Hänsler, R., León Fernández, O.S. & Fahmy, Z., 2016. Ozone in Medicine: Clinical Evaluation and Evidence Classification of the Systemic Ozone Applications, Major Autohemotherapy and Rectal Insufflation, According to the Requirements for Evidence-Based Medicine. Ozone: Science & Engineering, 38(5), pp.322–345. Available at: httpss://www.tandfonline.com/doi/full/10.1080/01919512.2016.1191992.
Watson, J.D., 2014. Type 2 diabetes as a redox disease. The Lancet, 383(9919), pp.841–843. Available at: https://linkinghub.elsevier.com/retrieve/pii/S014067361362365X.
Hybertson, B.M. et al., 2011. Oxidative stress in health and disease: The therapeutic potential of Nrf2 activation. Molecular Aspects of Medicine, 32(4-6), pp.234–246. Available at: https://linkinghub.elsevier.com/retrieve/pii/S0098299711000501.
Bocci, V. & Valacchi, G., 2015. Nrf2 activation as target to implement therapeutic treatments. Frontiers in Chemistry, 3. Available at: https://journal.frontiersin.org/Article/10.3389/fchem.2015.00004/abstract.
Bocci, V.A., 2006. Scientific and Medical Aspects of Ozone Therapy. State of the Art. Archives of Medical Research, 37(4), pp.425–435. Available at: https://linkinghub.elsevier.com/retrieve/pii/S0188440905003425.
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