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Rectal Ozone Insufflation

Rectal Ozone Insufflation is listed within the clinical range of evidence based medicine.

Safety of Rectal  Ozone O3 treatments.

In a controlled study in Germany 716 patients were treated with 46,984 ozone insufflations with no system or product related incidents or adverse reactions. Only in patients with severe IBS a few minor transient irritations in the form of flatulence have been observed.  

Based on the results and evidence of the study outcome Rectal Ozone Insufflation was listed within the clinical range of evidence based medicine. 

Ozone Concentration and Dosage

Based on the results of fundamental research over the past 18 years the ozone concentrations and required total amounts can be given in concrete terms. The concentrations listed below are cited in the measurements 1 mg/l. 

Rectal ozone concentrations for systemic preventative and immune supporting applications are recommended with a concentration of 10 to 40 mg/l with a volume of 150 – 300 ml for adults and 10 – 30 ml for children. 

Only for the treatment of ulcerative colitis, haemorrhages, haemorrhoids, proctitis stage 1 and 2,  anal fistulae, rectal, prostrate and colon cancer concentrations of 70 – 90 mg/l  are recommended with small volumes of 50ml 3 – 5 times on a daily basis. This is reduced to 27mg/l with 100 – 300 ml volumes for maintenance 2 – 3 times per week.  

As per Dr. Rau from the Paracelsus clinic in Swiss, Prostate cancer is always connected with long term bacteria infections  which are directly treated with ozone. Rectal ozone stimulates the immune system, provides plentiful oxygen to the immune cells for the respiratory burst and therefore increases chances of a recovery. Dr. Robert Rowen MD has also stunning results using Ozone rectally. 

Below you find a list of indications with best results based on clinical research for  rectal ozone insufflation. This list has been provided by Dr. Fiban Henzler. 


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Vaginal Insufflations

vaginal ozone insufflation

Vaginal Insufflations are just as effective and sometimes better tolerated than rectal insufflations. You can use the same Cuba protocol, but increase the volume of released ozone per treatment. Some of the doctors we asked for an opinion recommended up to 1400 ml per treatment. That equals 2 full insufflation bags. (Recommended concentration 16mg/l to 23mg/l)

You can even connect the catheter directly to the generator and apply the Ozone directly. Use a cloth or moist towel over your lab to prevent the inhalation of ozone.

Before starting with vaginal insufflation 100ml of ozonated water (administered via a syringe) must be instilled. This is extremely important and must not be forgotten. The vaginal mucosa is very thin and sensitive and needs to be moistened before the oxygen-ozone gas is applied.

Some doctors recommend to repopulate the vaginal flora with Lactobacillus probiotics vaginal tablets.



If you need to transport Ozone please consider the decomposition chart to the right. You can roughly say that O3 decomposes in 2 hours to half the value. So when you create a concentration of 80mg/l you have in 2 hours around 40mg/l.  But only if you keep the Ozone in the insufflation bag covered with ice chips in a cooling container or keep it in a fridge.  As soon as you reach room temperature the concentration dissolves back into O2 and water/condensation.

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I am not sure if we can use the keywords from the disease application list above for this page. 


16 Responses

  1. Hi There – I am hoping you can clear something up for me.

    I have a tumor in my lower colon and need to clarify the dose.

    Above it says this….

    Only for the treatment of ulcerative colitis, haemorrhages, haemorrhoids, proctitis stage 1 and 2, anal fistulae, rectal, prostrate and colon cancer concentrations of 70 – 90 mg/l are recommended with small volumes of 50ml 3 – 5 times on a daily basis. This is reduced to 27mg/l with 100 – 300 ml volumes for maintenance 2 – 3 times per week. My question is – how long do I do the 3-5 times daily? Can I overdo it? I’ve been doing it twice/day for about 4 days and plan to increase to 3 times/day.

    Then the chart reads as though it is only done 3-5 times – then move to CUBA protocol.

    Could you please clarify?

    Thanks for ll your wonderful work very much appreciated.

  2. David Sondergeld says:

    I am interested in flooding my body with oxygen but do not want to be so aggressive as to cause any harm. I know, I have heard you say not to do IV ozone AND RI ozone on the same day. I have been doing 200-300ccs of ozone on setting 7 every day gradually increasing until I get up to 400 cc. I will begin EWOT (Exercise With Oxygen Therapy on Monday for 5-15 minutes breathing 94% pure oxygen. I am somewhat concerned about developing oxygen toxicity, however so few physicians are familiar with both EWOT and RI ozone it is almost impossible to get any answer… I have underlying COPD along with a small <2cm nodule squamous cell cancer of the lung. I am also considering bagging, since the nodule is located posterior against the rib wall of my upper lung field.

  3. Hi Marcus, How long should the ozone be held in the colon before you can let go and would the time increase with volume?

    1. You can let it go immediately. I first said that you should hold it as long as you can but thats not necessary. The effects happen when you insert the gas immediately.

      1. Hi Marcus,

        I read that Cuban scientists recently proved rectal ozone is absorbed almost immediately by the intestinal wall and that you’ll just be releasing oxygen afterwards since the ozone component is absorbed so quickly.

        But what I’m having a hard time understanding is, if this is the case why is the smell of ozone stronger when released at let’s say 2 minutes versus 15 minutes of hold time? If the ozone component is absorbed immediately it seems there would be no ozone smell, but only the smell of oxygen, when later being released.

        Don’t get me wrong. I’m not trying to disagree but only to understand.

        1. Hello Scott, first off I have actually tried to avoid smelling my farts after Ozone treatment and not attempted to evaluate the intensity of the smell. Funny. Never thought of it. Ozone smells very intensely even in tiny quantities and seems to seep into any material. After using a treatment bag the bag itself smells for a long time like Ozone. I assume that you mistake the sulphurous smell of dying bacteria as Ozone smell. It’s just as pungent and intense.

          Also remember that the main effect does not come from the immediate treatment but the chain reactions triggered by the treatment. This short 4 second oxidation of a few bacteria is not the treatment effect. It’s the beginning of a chain reaction that up-regulates – your self healing, – your antioxidant production and your cell defence. The effects that happen after the treatment are the key to your health.

  4. Hi Marcus, The 60ml syringes say single use on the pack, are these safe for multiple use with ozone?

    1. Single use for blood or any medium. ozone is self sterilising but the rubber slowly disintegrates. We use them around 5 to 6 times with gas and then get a new one. You can get them cheap on Ebay or you can order a glass syringe and use it for a long time.

  5. Lima Dabbouk says:

    Hi Marcus, been diagnosed with stage 4 breast cancer and plan on doing ozone daily. I have tested the 70mg/l to see if it irritates me and it doesn’t. I did chemo 6 months ago and i take a table every day which is a form of chemo but in a small dose (palbocibl). My question is to systematically boost my immune system 200ml seems like a small dosage. Your opinion would be greatly appreciated.

    1. Hello Lima, 200ml is just fine. More does NOT help more when you do rectal insufflations. It’s a switch that is turned on with 60ml and 200ml just alike.

      It’s different if you do DIV or Hyperbaric ozone as the amount of ozone they use, sterilises the blood at the same time while triggering the immune boost effect. It’s kind of 2 treatment at once and especially helpful ifs you have chronic infections.

  6. Carlos Romero. says:


    Do you leave or take out the water that you installed in the vagina during the vaginal insuflation?

    1. I assume most will run out into a towel as soon as you sit up. You can use afterwards an inlay in your undies to prevent walking around with wet pants.

  7. What treatment do you reommend for HSV?

  8. Hi Marcus, What is the protocol for an autistic toddler please?

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